RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols

Abstract

The goal of this book is to provide an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. We believed this was a noteworthy goal because the fields of RNAi, miRNA, and stem cell biology have been undergoing explosive growth, which makes it difficult for anyone to keep abreast of the seminal methods used to conduct experiments and analyze results. We recognized that the availability of detailed, user-tested protocols for conducting RNAi and small RNA-based research on stem cells would accelerate progress in the field by reducing the time required to decipher and put into practice procedures published in the literature.