Heterologous Gene Expression in E. coli: Methods and Protocols

Abstract

This book is aimed at molecular biologists, biochemists, and structural biologists, both from the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism. The topics are largely grouped under four parts: (I) highthroughput cloning, expression screening, and optimization of expression conditions, (II) protein production and solubility enhancement, (III) case studies to produce challenging proteins and specifc protein families, and (IV) applications of E. coli expression. This volume provides scientists with a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and innovative engineering of E. coli. It will hopefully prove valuable both in small laboratories and in higher throughput facilities. I would like to thank all the authors for their contributions and for making this a global effort.