Please use this identifier to cite or link to this item: http://thuvien.ued.udn.vn/handle/TVDHSPDN_123456789/57180
Title: Heterologous Gene Expression in E. coli: Methods and Protocols
Authors: Burgess-Brown, Nicola A., ed.
Keywords: Methods in Molecular Biology
Heterologous gene
Issue Date: 2017
Publisher: Humana Press
Abstract: This book is aimed at molecular biologists, biochemists, and structural biologists, both from the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism. The topics are largely grouped under four parts: (I) highthroughput cloning, expression screening, and optimization of expression conditions, (II) protein production and solubility enhancement, (III) case studies to produce challenging proteins and specifc protein families, and (IV) applications of E. coli expression. This volume provides scientists with a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and innovative engineering of E. coli. It will hopefully prove valuable both in small laboratories and in higher throughput facilities. I would like to thank all the authors for their contributions and for making this a global effort.
URI: http://thuvien.ued.udn.vn/handle/TVDHSPDN_123456789/57180
ISBN: 978-1-4939-6887-9
Appears in Collections:Biology

Files in This Item:

 Sign in to read

If the system does not display after logging in, please press F5 to refresh.



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Google Scholar TM

Check...